Journal: BioMed Research International

Article Title: DNA Methylation Pyrosequencing Assay Is Applicable for the Assessment of Epigenetic Active Environmental or Clinical Relevant Chemicals

doi: 10.1155/2013/486072

Figure Lengend Snippet: Human methylation positive and negative controls (EpigenDX, USA) and PyroMark LINE1 kit (Qiagen, Germany).

Article Snippet: The assays were proofed using human methylation positive and negative controls as seen in (ordered at EpigenDX, USA).

Techniques: Methylation

Journal: BioMed Research International

Article Title: DNA Methylation Pyrosequencing Assay Is Applicable for the Assessment of Epigenetic Active Environmental or Clinical Relevant Chemicals

doi: 10.1155/2013/486072

Figure Lengend Snippet: Established tumor, nontumor, and primary cell lines investigated for their intrinsic methylation level of the LINE1 element (Qiagen, Germany): CKA-CA6 kidney carcinoma; HEK293 human embryonic kidney cells, spontaneously immortalized human keratinocytes (HACAT), and primary in vitro cultures of normal human dermal fibroblasts (NHDF).

Article Snippet: The assays were proofed using human methylation positive and negative controls as seen in (ordered at EpigenDX, USA).

Techniques: Methylation, In Vitro

Journal: BioMed Research International

Article Title: DNA Methylation Pyrosequencing Assay Is Applicable for the Assessment of Epigenetic Active Environmental or Clinical Relevant Chemicals

doi: 10.1155/2013/486072

Figure Lengend Snippet: Quantitation of intrinsic methylation level of the LINE1 element (Qiagen, Germany) in established tumor, nontumor, and primary cell lines using several measurement replicates ( n = 3–6) and the correspondent methylation controls (Student's t -test, * P < 0.05).

Article Snippet: The assays were proofed using human methylation positive and negative controls as seen in (ordered at EpigenDX, USA).

Techniques: Quantitation Assay, Methylation

Journal: BioMed Research International

Article Title: DNA Methylation Pyrosequencing Assay Is Applicable for the Assessment of Epigenetic Active Environmental or Clinical Relevant Chemicals

doi: 10.1155/2013/486072

Figure Lengend Snippet: Glioma cell lines were investigated for the methylation level of LINE1 element (Qiagen, Germany) for LNT 229, LN 18, and LNT 308. Temozolomide resistant strains of same cell lines that were obtained with treatment of increasing concentrations of the drug; exemplarily depicted is LNT 308 P (drug sensitive) and LNT 308 R (drug resistant).

Article Snippet: The assays were proofed using human methylation positive and negative controls as seen in (ordered at EpigenDX, USA).

Techniques: Methylation

Journal: BioMed Research International

Article Title: DNA Methylation Pyrosequencing Assay Is Applicable for the Assessment of Epigenetic Active Environmental or Clinical Relevant Chemicals

doi: 10.1155/2013/486072

Figure Lengend Snippet: Quantitation of intrinsic methylation level of the LINE1 element (Qiagen, Germany) in Temozolomide sensitive and resistant glioma cell lines using several measurement replicates ( n = 3–6) and the correspondent methylation controls (Student's t -test, * P < 0.05).

Article Snippet: The assays were proofed using human methylation positive and negative controls as seen in (ordered at EpigenDX, USA).

Techniques: Quantitation Assay, Methylation

Journal: BioMed Research International

Article Title: DNA Methylation Pyrosequencing Assay Is Applicable for the Assessment of Epigenetic Active Environmental or Clinical Relevant Chemicals

doi: 10.1155/2013/486072

Figure Lengend Snippet: Assessment of HACAT cells methylation after in vitro chemical treatment using the self-designed assays (PSQ primer design, Qiagen, Germany). HACAT cells were treated in vitro with DNA demethylating agent 5-aza-2′-deoxycytidine (A) (1 μ M for 72 h) and the histone deacetylase inhibitor trichostatin A (T2) (1 μ M for 36 h).

Article Snippet: The assays were proofed using human methylation positive and negative controls as seen in (ordered at EpigenDX, USA).

Techniques: Methylation, In Vitro, Histone Deacetylase Assay

Journal: BioMed Research International

Article Title: DNA Methylation Pyrosequencing Assay Is Applicable for the Assessment of Epigenetic Active Environmental or Clinical Relevant Chemicals

doi: 10.1155/2013/486072

Figure Lengend Snippet: Assessment of HACAT cells methylation after in vitro chemical treatment using LINE1 element kit (Qiagen, Germany). HACAT cells were treated in vitro with DNA demethylating agent 5-aza-2′-deoxycytidine (A) (1 μ M for 72 h) and the histone deacetylase inhibitor trichostatin A (T2) (1 μ M for 36 h).

Article Snippet: The assays were proofed using human methylation positive and negative controls as seen in (ordered at EpigenDX, USA).

Techniques: Methylation, In Vitro, Histone Deacetylase Assay